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《医学前沿(英文)》 2022年 第16卷 第4期 页码 584-595 doi: 10.1007/s11684-021-0844-7
关键词: hemophilia A adeno-associated virus (AAV) human/rat hybrid factor VIII gene therapy dual chain strategy
null
《医学前沿(英文)》 2012年 第6卷 第4期 页码 411-415 doi: 10.1007/s11684-012-0226-2
Ketamine exerts rapid and robust antidepressant properties in both animal models and depressed patients and tramadol possesses potential antidepressant effects. Brain-derived neurotrophic factor (BDNF) is an important biomarker for mood disorders and tropomyosin-related kinase B (TrkB) is a high affinity catalytic receptor for BDNF. We hypothesized that tramadol pretreatment might reinforce ketamine-elicited antidepressant effects with significant changes in hippocampal BDNF and TrkB levels in rats. Immobility time of rats receiving different treatment in the forced swimming test (FST) was observed. Levels of BDNF and TrkB in hippocampus were measured by enzyme linked immunosorbent assay. Results showed that tramadol (5 mg/kg) administrated alone neither elicited antidepressant effects nor altered BDNF or TrkB level. However, pretreatment with tramadol (5 mg/kg) enhanced the ketamine (10 mg/kg) -elicited antidepressant effects and upregulated the BDNF and TrkB levels in hippocampus. In conclusion, tramadol pretreatment reinforces the ketamine-elicited antidepressant effects, which is associated with the increased levels of BDNF and TrkB in rat hippocampus.
关键词: tramadol ketamine antidepressant brain-derived neurotrophic factor tropomyosin-related kinase B
Hepatitis B virus X protein upregulates tumor necrosis factor-α expression of rat mesangial cell
Hong-Zhu LU MD, Dan LIU BM, Qi-Hong FAN BM, Jian-Hua ZHOU MD,
《医学前沿(英文)》 2010年 第4卷 第1期 页码 106-111 doi: 10.1007/s11684-010-0004-y
关键词: hepatitis B virus X gene glomerular mesangial cell line extracellular regulated protein kinases tumor necrosis factor-α
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
null
《医学前沿(英文)》 2015年 第9卷 第1期 页码 90-99 doi: 10.1007/s11684-015-0390-2
Gene therapy provides a potential cure for hemophilia B, and significant progress has been achieved in liver-directed gene transfer mediated by adeno-associated viral vectors. Recent clinical trials involving the use of a self-complementary adeno-associated virus serotype 8-human codon-optimized factor IX (AAV8-hFIXco) vector demonstrated encouraging efficacy with hFIX expression stabilized at 1% to 6% of normal level in patients, but safety concerns related to high vector doses are still present. Thus, further improvement of AAV vectors and hFIX expression cassette may positively contribute to the ultimate success of hemophilia B gene therapy. In this study, to obtain a higher expression level of hFIX that potentiates the coagulant capacity of recipients, human FIX expression vector was optimized by upgrading the codon adaption index and adjusting the GC content, inserting a Kozak sequence (GCCACC), and introducing a gain-of-function mutation, R338L (FIX Padua). The efficiency of the published and the presently constructed cassettes was compared through in vivo screening. In addition, the regulatory elements that control the FIX gene expression in these cassettes were screened for liver-specific effectiveness. Among all the constructed cassettes, scAAV-Pre-hFIXco-SIH-R338L, which was the construct under the control of the prothrombin enhancer and prealbumin promoter, resulted in the highest level of coagulant activity, and the expression levels of two constructed cassettes (scAAV-Chi-hFIXco-SIH-R338L and scAAV-Pre-hFIXco-SIH-R338L) were also higher than that of the published cassette (scAAV-LP1-hFIXco-SJ). In summary, our strategies led to a substantial increase in hFIX expression at the protein level or a remarkably elevated coagulant activity. Thus, these reconstructs of hFIX with AAV vector may potentially contribute to the creation of an efficacious gene therapy of hemophilia B.
关键词: factor IX hemophilia B liver-specific regulatory elements hydrodynamic gene transfer
662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)
QU Yanchun, YANG Ze, SUN Liang, JI Linong
《医学前沿(英文)》 2008年 第2卷 第3期 页码 283-285 doi: 10.1007/s11684-008-0053-7
关键词: D-HPLC mutation development autoimmune PCR-RFLP candidate
Introduction to the special issue on the VIII Latin-American Congress on Mechanical Engineering
Marco CECCARELLI,René-Vinicio SÁNCHEZ
《机械工程前沿(英文)》 2015年 第10卷 第3期 页码 219-220 doi: 10.1007/s11465-015-0356-8
Wenjie Zhu, Binghe Xu
《医学前沿(英文)》 2021年 第15卷 第2期 页码 208-220 doi: 10.1007/s11684-020-0795-4
关键词: endocrine-resistant HR+/HER2- advanced breast cancer randomized clinical trials meta-analysis targeted therapy
Bin YANG MS , Xianglin YUAN , Yanmei ZOU , Qingsong XI , Guoxian LONG , Qiang FU , Guangyuan HU MM ,
《医学前沿(英文)》 2009年 第3卷 第3期 页码 303-308 doi: 10.1007/s11684-009-0060-3
c-Fos expression in rat brainstem following intake of sucrose or saccharin
Ke Chen, Jianqun Yan, Jinrong Li, Bo Lv, Xiaolin Zhao
《医学前沿(英文)》 2011年 第5卷 第3期 页码 294-301 doi: 10.1007/s11684-011-0144-8
关键词: c-Fos parabrachial the nucleus of the solitary tract sweet tastant rat
Mengjie LI, Chunbao LI, Shangxin SONG, Xinglian XU, Guanghong ZHOU
《农业科学与工程前沿(英文)》 2018年 第5卷 第3期 页码 362-372 doi: 10.15302/J-FASE-2018206
This study compared proteome profiles and morphological changes of rat jejunum in response to different dietary proteins. Fifty male Sprague-Dawley rats were fed with casein (control), and isolated beef, pork, fish and chicken proteins for 14 days. Proteome analysis, histological observation and PEPT1 quantification of the jejunum were performed. The results indicated that rats fed with chicken proteins had higher PEPT1 mRNA and protein levels (P<0.05) but lower villus height and ratio of villus height to crypt depth (V/C ratio, P<0.05) than those fed with casein and pork protein. Label-free LC-MS/MS indicated that, as compared to casein, intake of chicken protein can regulate oligopeptide transport mainly by upregulating PEPT1 protein expression and reducing dipeptidyl-peptidase activity related to biological oxidation, and can reduce oligopeptide absorption capacity by regulating Hippo signaling pathway. Although intake of beef and fish proteins had no significant effect on PEPT1 expression, they altered several signaling pathways.
关键词: Hippo signaling pathway meat protein PEPT1 proteome analysis rat jejunum
A modified chronic ocular hypertension rat model for retinal ganglion cell neuroprotection
null
《医学前沿(英文)》 2013年 第7卷 第3期 页码 367-377 doi: 10.1007/s11684-013-0266-2
This study aimed to modify a chronic ocular hypertension (OHT) rat model to screen for potential compounds to protect retinal ganglion cells (RGCs) from responding to increased intraocular pressure (IOP). A total of 266 rats were prepared and randomly grouped according to different time-points, namely, weeks 3, 8, 16, and 24. Rats were sedated and eye examination was performed to score as the corneal damage on a scale of 1 to 4. The OHT rat model was created via the injection of a hypertonic saline solution into the episcleral veins once weekly for two weeks. OHT was identified when the IOP at week 0 was≥6 mmHg than that at week -2 for the same eye. Viable RGCs were labeled by injecting 4% FluoroGold. Rats were sacrificed, and the eyes were enucleated and fixed. The fixed retinas were dissected to prepare flat whole-mounts. The viable RGCs were visualized and imaged. The IOP (meanβ±βSD) was calculated, and data were analyzed by the paired t-test and one-way ANOVA. The OHT model was created in 234 of 266 rats (87.97%), whereas 32 rats (12.03%) were removed from the study because of the absence of IOP elevation (11.28%) and/or corneal damage scores over 4 (0.75%). IOP was elevated by as much as 81.35% for 24 weeks. The average IOP was (16.68β±β0.98)βmmHg in non-OHT eyes (n = 234), but was (27.95±0.97)βmmHg in OHT eyes (n = 234). Viable RGCs in the OHT eyes were significantly decreased in a time-dependent manner by 29.41%, 38.24%, 55.32%, and 59.30% at weeks 3, 8, 16, and 24, respectively, as compared to viable RGCs in the non-OHT eyes (P<β0.05). The OHT model was successfully created in 88% of the rats. The IOP in the OHT eyes was elevated by approximately 81% for 24 weeks. The number of viable RGCs was decreased by 59% of the rats in a time-dependent manner. The modified OHT model may provide an effective and reliable method for screening drugs to protect RGCs from glaucoma.
关键词: chronic ocular hypertension intraocular pressure retinal ganglion cells neuroprotection glaucoma
Qiong CHEN, Qing YU, Yuhu SONG, Peiyuan Li, Ying CHANG, Zhijun WANG, Lifeng LIU, Wei WU, Jusheng LIN
《医学前沿(英文)》 2009年 第3卷 第2期 页码 148-152 doi: 10.1007/s11684-009-0032-7
关键词: gene X-linked inhibitor of apoptosis protein associated factor-1 (XAF1) promoter transcription regulation
XIA Jiao, KONG Weijia, ZHU Yun, ZHOU Yan, ZHANG Yu, GUO Changkai
《医学前沿(英文)》 2008年 第2卷 第3期 页码 264-268 doi: 10.1007/s11684-008-0050-x
关键词: resistance infrared infrared videomicroscopy depressive after-hyperpolarization potential
Construction and identification of lentiviral RNA interference vector of rat leptin receptor gene
Zhengjuan LIU, Jie BIAN, Yuchuan WANG, Yongli ZHAO, Dong YAN, Xiaoxia WANG
《医学前沿(英文)》 2009年 第3卷 第1期 页码 57-60 doi: 10.1007/s11684-009-0003-z
Xiaoxiao XU, Patrick X. W. ZOU
《工程管理前沿(英文)》 2021年 第8卷 第1期 页码 17-31 doi: 10.1007/s42524-019-0091-7
关键词: system dynamics construction management problem and recommendation research directions literature review human factor
标题 作者 时间 类型 操作
Long-term correction of hemorrhagic diathesis in hemophilia A mice by an AAV-delivered hybrid FVIII composedof the human heavy chain and the rat light chain
期刊论文
Tramadol reinforces antidepressant effects of ketamine with increased levels of brain-derived neurotrophic factorand tropomyosin-related kinase B in rat hippocampus
null
期刊论文
Hepatitis B virus X protein upregulates tumor necrosis factor-α expression of rat mesangial cell
Hong-Zhu LU MD, Dan LIU BM, Qi-Hong FAN BM, Jian-Hua ZHOU MD,
期刊论文
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
null
期刊论文
662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)
QU Yanchun, YANG Ze, SUN Liang, JI Linong
期刊论文
Introduction to the special issue on the VIII Latin-American Congress on Mechanical Engineering
Marco CECCARELLI,René-Vinicio SÁNCHEZ
期刊论文
Overcoming resistance to endocrine therapy in hormone receptor-positive human epidermal growth factor
Wenjie Zhu, Binghe Xu
期刊论文
Effects of hypoxia inducible factor-1alpha siRNA on the invasion of human Hela cells and expression of
Bin YANG MS , Xianglin YUAN , Yanmei ZOU , Qingsong XI , Guoxian LONG , Qiang FU , Guangyuan HU MM ,
期刊论文
c-Fos expression in rat brainstem following intake of sucrose or saccharin
Ke Chen, Jianqun Yan, Jinrong Li, Bo Lv, Xiaolin Zhao
期刊论文
Proteome comparisons reveal influence of different dietary proteins on the development of rat jejunum
Mengjie LI, Chunbao LI, Shangxin SONG, Xinglian XU, Guanghong ZHOU
期刊论文
A modified chronic ocular hypertension rat model for retinal ganglion cell neuroprotection
null
期刊论文
Cloning of human XAF1 gene promoter and assay of its transcription activity in a variety of cell lines
Qiong CHEN, Qing YU, Yuhu SONG, Peiyuan Li, Ying CHANG, Zhijun WANG, Lifeng LIU, Wei WU, Jusheng LIN
期刊论文
Spontaneous firing properties of rat medial vestibular nucleus neurons in brain slices by infrared visual
XIA Jiao, KONG Weijia, ZHU Yun, ZHOU Yan, ZHANG Yu, GUO Changkai
期刊论文
Construction and identification of lentiviral RNA interference vector of rat leptin receptor gene
Zhengjuan LIU, Jie BIAN, Yuchuan WANG, Yongli ZHAO, Dong YAN, Xiaoxia WANG
期刊论文